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DETECTION OF CHLORAMPHENICOL RESIDUES IN MILK SAMPLES USING ELISA AND LC/MS-MS
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Author
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Fatmira SHEHU
Enkela ZANI, Suzana KOLA, Ermira MARKU, Bizena BIJO
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2018
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3
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Fatmira SHEHU Enkela ZANI, Suzana KOLA, Ermira MARKU, Bizena BIJO, (2018). DETECTION OF CHLORAMPHENICOL RESIDUES IN MILK SAMPLES USING ELISA AND LC/MS-MS. EJONS INTERNATIONAL JOURNAL ON MATHEMATICS, ENGINEERING - NATURAL SCIENCES, 3, p. . Doi: .
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Summary
The use of Chloramphenicol in food producing animals is prohibited in many countries including the United States, Canada, the European Union, and Australia due to the high potential risk of severe effects such as aplastic anemia, allergic reactions, and the promotion of antibiotic resistance. The monitoring of food products, such as milk, meat and honey, for antibiotic residues is necessary to ascertain that these compounds are not misused and do not present a danger to human or animal health. 244 milk samples were collected in 12 regions of Albania during 2017. In this study, an analytical method for screening and confirmation of CAP residues in milk samples is described. The ELISA was carried out to screen milk samples, and LC/MSMS was applied to confirm suspect samples. Through the use of Elisa, the presence of Choramphenicol residues was found in 4 milk samples. These samples subsequently subjected the test to the confirmatory method, which resulted in: 2 positive and 2 false positive samples. The method has been validated according to the criteria of the 2002/657/EC Decision
Keywords
Chloramphenicol, ELISA, milk, LC-MS/MS
Abstract
The use of Chloramphenicol in food producing animals is prohibited in many countries including the United States, Canada, the European Union, and Australia due to the high potential risk of severe effects such as aplastic anemia, allergic reactions, and the promotion of antibiotic resistance. The monitoring of food products, such as milk, meat and honey, for antibiotic residues is necessary to ascertain that these compounds are not misused and do not present a danger to human or animal health. 244 milk samples were collected in 12 regions of Albania during 2017. In this study, an analytical method for screening and confirmation of CAP residues in milk samples is described. The ELISA was carried out to screen milk samples, and LC/MSMS was applied to confirm suspect samples. Through the use of Elisa, the presence of Choramphenicol residues was found in 4 milk samples. These samples subsequently subjected the test to the confirmatory method, which resulted in: 2 positive and 2 false positive samples. The method has been validated according to the criteria of the 2002/657/EC Decision
Keywords
Chloramphenicol, ELISA, milk, LC-MS/MS
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