EJONS INTERNATIONAL JOURNAL ON MATHEMATICS, ENGINEERING & NATURAL SCIENCES ISSN 2602 - 4136

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OCCURRENCE OF AFLATOXIN M1 IN ALBANIAN RAW MILK DETECTED BY ELISA TECHNIQUE
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Author : Fatmira SHEHU  Enkela ZANI, Suzana KOLA, Ermira MARKU, Bizena BIJO  
Type :
Printing Year : 2018
Number : 3
Page :
Cite : Fatmira SHEHU Enkela ZANI, Suzana KOLA, Ermira MARKU, Bizena BIJO, (2018). OCCURRENCE OF AFLATOXIN M1 IN ALBANIAN RAW MILK DETECTED BY ELISA TECHNIQUE. EJONS INTERNATIONAL JOURNAL ON MATHEMATICS, ENGINEERING - NATURAL SCIENCES, 3, p. . Doi: .
    


Summary


Keywords


Abstract

AFM1 (Aflatoxin M1) is the major mycotoxin frequently found in milk and dairy products. Tolerance limits for aflatoxins in dairy products have been established internationally. According to the requirements of EU Regulation (EC) No.401 / 2006 the maximum residue level (EU MRL) of AFM1 is 50 ng/kg for milk and dairy products. The purpose of this study was to detect the occurrence and determinate the presence of AFM1 milk In Albania, there are scarce published data about AFM1 level in dairy products and none of the consumer exposure to this toxin. A total of 180 milk samples were collected in January- December 2016, from 13 small farms of Myzeqeja region in Albania. Samples were analyzed for the presence of AFM1 content using competitive ELISA (RIDASCREEN R-biopharm test kit). The competitive enzyme immunoassay- Ridascreen method highlighted the contamination with AFM1 in 98/180 milk samples. Beside to the high number of samples we detected huge level of concentration. AFM1 levels oscillated from 3.25 - 131 ng / L, where one of the most important factor affected was considered the season of the year. Results demonstrated a significant (p < 0.05) difference of the AFM1 content in milk samples between autumn and spring seasons. Milk samples collected in February and March presented a high positivity of and the highest AFM1 level (131 ng / L).



Keywords
raw milk, aflatoxin M1, ELISA, public health

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